The London-born midfielder, 20, has recently switched allegiance from the Republic of Ireland to England. A move which has been approved by world governing body FIFA as all three matches played by Rice for the Republic were friendly appearances.
England host the Czechs on 22 March and are away in Montenegro on 25 March.
Goalkeepers: Jack Butland (Stoke City), Tom Heaton (Burnley), Jordan Pickford (Everton)
Defenders: Trent Alexander-Arnold (Liverpool), Ben Chilwell (Leicester City), Michael Keane (Everton), Harry Maguire (Leicester City), Danny Rose (Tottenham Hotspur), Luke Shaw (Manchester United), John Stones (Manchester City), James Tarkowski (Burnley), Kieran Trippier (Tottenham Hotspur), Kyle Walker (Manchester City)
Midfielders: Ross Barkley (Chelsea), Dele Alli (Tottenham Hotspur), Fabian Delph (Manchester City), Eric Dier (Tottenham Hotspur), Jordan Henderson (Liverpool), Ruben Loftus-Cheek (Chelsea), Declan Rice (West Ham)
Forwards: Harry Kane (Tottenham Hotspur), Marcus Rashford (Manchester United), Jadon Sancho (Borussia Dortmund), Raheem Sterling (Manchester City), Callum Wilson (Bournemouth